University of Otago.Department of Physiology.Department of Physiology.

Hamilton Lab

Dr Kirk Hamilton

Kirk Hamilton

Senior Lecturer

Department of Physiology
Otago School of Medical Sciences
University of Otago
PO Box 913
Dunedin
New Zealand

Phone: 4797252
Fax: +64 3 479 7323

Email: kirk.hamilton@otago.ac.nz

Member of Membrane & Ion Transport.

Member of The Kidney in Health and Disease Research Theme, Auckland Kidney Physiome Project - Auckland Bioengineering Institute and Gut Health Network.

Research interests

My main research interest is the molecular physiology, regulation and function of membrane ion channels of epithelial tissues. I have had a long-term interest in the molecular physiology and pharmacology of epithelial ion channels and K+ channels, specifically, the Ca2+-dependent, intermediate-conductance K+ channel, KCa3.1. Recently, my lab has been examining the anterograde and retrograde trafficking of KCa3.1 to and from the basolateral membrane of polarized epithelia. The cytoskeleton and accessory proteins play major roles in the trafficking of proteins throughout the cell and to and from the membrane. Additoinally, protein complexes such as the Exocyst and Retromer and SNARE proteins play significance roles in proper transport and targeting of ion channels and transporters to the appropriate membranes of epithellial cells.

We are using biotin-ligase acceptor peptide (BLAP) technology to tagged K+ channels in order to examine their cellular and membrane fate. This technique has allowed us to ask many important questions about the molecular physiology and trageting of KCa3.1 to the basolateral membrane of epithelial cells.

I employ electrophysiological (patch-clamp and short circuit current measurements) and molecular biochemical techniques (immunofluorescence, immunoprecipitation, co-immunoprecipitation, cell surface immunoprecipitation, gel electrophoresis, biotinylation, PCR) to address basic questions of cell physiology and biology of ion channels.

Research topics

Current funding

Previous funding

Collaborators

Lab personnel

Joshua April

Joshua April

Elliot Pilmore

Elliot Pilmore

Monique Scott

Monique Scott


Previous students

Selected publications

Edited Book

Hamilton, K.L. (Editor) and Devor, D.C. (Co-Editor) Ion Channels and Transporters in Health and Disease. 1st Edition, Physiology in Health and Disease Series. 29 Chapters, 1,019 pp, Springer-Verlag (New York) , ISBN 978-1-4939-3364-8, 2016.

Journal publications

Book chapters

Teaching

Joshua April

Joshua April

PGDipSci Student

Department of Physiology
Otago School of Medical Sciences
University of Otago
PO Box 913
Dunedin
New Zealand

Phone: 479 7316
Fax: +64 3 479 7323

Email: aprjo818@student.otago.ac.nz

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Elliot Pilmore

Elliot Pilmore

MSc Student

Department of Physiology
Otago School of Medical Sciences
University of Otago
PO Box 913
Dunedin
New Zealand

Phone: 479 7316
Fax: +64 3 479 7323

Email: pilel408@student.otago.ac.nz

Show Hamilton Lab page.

Monique Scott

Monique Scott

PGDipSci

Department of Physiology
Otago School of Medical Sciences
University of Otago
PO Box 913
Dunedin
New Zealand

Phone: 479 7316
Fax: +64 3 479 7323

Email: scomo001@student.otago.ac.nz

Show Hamilton Lab page.

I really see no harm which can come of giving our children a little knowledge of physiology.

Thomas Henry Huxley - Science and Culture (1882), 92