Dr Kirk Hamilton
Department of Physiology
Otago School of Medical Sciences
University of Otago
PO Box 913
Fax: +64 3 479 7323
Member of Membrane & Ion Transport.
Member of The Kidney in Health and Disease Research Theme, Auckland Kidney Physiome Project - Auckland Bioengineering Institute and Gut Health Network.
My main research interest is the molecular physiology, regulation and function of membrane ion channels of epithelial tissues. I have had a long-term interest in the molecular physiology and pharmacology of epithelial ion channels and K+ channels, specifically, the Ca2+-dependent, intermediate-conductance K+ channel, KCa3.1. Recently, my lab has been examining the anterograde and retrograde trafficking of KCa3.1 to and from the basolateral membrane of polarized epithelia. The cytoskeleton and accessory proteins play major roles in the trafficking of proteins throughout the cell and to and from the membrane. Additoinally, protein complexes such as the Exocyst and Retromer and SNARE proteins play significance roles in proper transport and targeting of ion channels and transporters to the appropriate membranes of epithellial cells.
We are using biotin-ligase acceptor peptide (BLAP) technology to tagged K+ channels in order to examine their cellular and membrane fate. This technique has allowed us to ask many important questions about the molecular physiology and trageting of KCa3.1 to the basolateral membrane of epithelial cells.
I employ electrophysiological (patch-clamp and short circuit current measurements) and molecular biochemical techniques (immunofluorescence, immunoprecipitation, co-immunoprecipitation, cell surface immunoprecipitation, gel electrophoresis, biotinylation, PCR) to address basic questions of cell physiology and biology of ion channels.
- Molecular physiology of channels
- Cellular and membrane trafficking of ion channels
- The role of the Exocyst complex in the trafficking ion channels
- SNARE protein regulation of ion channels via protein-protein interactions
- The role of Retromer in the trafficking ion channels
- Dean’s Bequest Fund, University of Otago, 2017
- Department of Physiology Aims Grant, 2017
- Lottery Grants Board, 2016-2017 (with A/P Fiona McDonald)
- Department of Physiology Aims Grant, 2016
- University of Otago Research Grant, 2015 (with A/P Fiona McDonald)
- Dean’s Bequest Fund, University of Otago, 2014-2015
- University of Otago Research Grant, 2013-2014
- OSMS Strategic Grant, 2013-2014
- Prof. Yoshi Marunaka, Department of Molecular Cell Physiology Graduate School of Medical Science, Kyoto Prefectural University of Medicine, Kyoto, Japan,
- Prof. Daniel C. Devor, Department of Cell Biology and Physiology, School of Medicine, University of Pittsburgh, PA, USA
- Prof. Peter Hunter, and Dr. David Nickerson, Auckland Bioengineering Institute, University of Auckland, NZ
- Dr. Michael Butterworth, Department of Cell Biology and Physiology, School of Medicine, University of Pittsburgh, PA, USA
- A/Prof Fiona J. McDonald, Department of Physiology, University of Otago, NZ
- A/Prof Antoni Moore, Department of Surveying, University of Otago, NZ
- Dr Steven B. Condliffe, Department of Physiology, University of Otago, NZ, (deceased)
- Rachel Farquhar (BBiomedSc and MSc) - now an ARF with Prof Jamie Rossjohn, Department of Biochemistry and Molecular Biology, Monash University, Australia
- Sibi Narayanan (PGDipSci)
- Shih-Liang (Bob) Lee (MSc) - currently PhD candidate at the School of Medicine, University of Sydney, Australia
Hamilton, K.L. (Editor) and Devor, D.C. (Co-Editor) Ion Channels and Transporters in Health and Disease. 1st Edition, Physiology in Health and Disease Series. 29 Chapters, 1,019 pp, Springer-Verlag (New York) , ISBN 978-1-4939-3364-8, 2016.
- • Lee, S.L., Devor, D.C., and Hamilton, K.L. Modulation of retrograde trafficking of KCa3.1 in a polarized epithelium. Frontiers of Physiology, 8:489 doi: 10.3389/fphys.2017.00489, 11 pages, (2017)
- Farquhar, R.E., Rodrigues, E., and Hamilton, K.L. The role of the cytoskeleton and Myosin-Vc in the trafficking of KCa3.1 to the basolateral membrane of polarized epithelial cells. Frontiers of Physiology, 7:639, doi:10.3389/fphys.2016.00639, 12 pages, (2017)
- Hamilton, K.L. and Moore A.B. 50 years of renal physiology from one man and the isolate perfused tubule: Maurice B. Burg. American Journal Physiology – Renal Physiology 311:F291-F304, (2016)
- Hamilton, K.L. New Life in Overactive Bladder: Focus on “Novel regulatory Mechanism in human urinary bladder: Central role of TRPM4 channels in detrusor smooth muscle function”. American Journal Physiology – Cell Physiology 310:C597-C599, (2016)
- Pilmore, E., and Hamilton, K.L. The role of microRNAs in the regulation of K+ channels in epithelial tissue. Frontiers in Physiology 6:352. doi: 10.3389/fphys.2015.00352, 7 pages, (2015)
- Hamilton, K.L. Cleavage – What’s new with prostasin and ENaC these days? American Journal Physiology – Renal Physiology, 307:1196-1197. (2014).
- Hamilton, K.L. Even an old technique is suitable in the modern molecular world of Science: The everted sec preparation – Turns 60 years old. Amer. J. Physiol. Cell Physiol, 306:C715-C720. (2014).
- Sun, H, Niisato, N, Nishio K, Hamilton, K.L. and Marunaka, Y. Distinct action of flavonoids, myricetin and quercetin, on epithelial Cl- secretion: useful tools as regulators of Cl- secretion. Biomed. Res. Int., Vol. 2014, Article ID 902735, 8 pages. (2014).
- Bertuccio, C.A., Lee, S.-L., Wu, G., Butterworth, M.B., Hamilton*, K.L. and Devor*, D.C. Anterograde trafficking of KCa3.1 in polarized epithelia is Rab1- and Rab8-dependent and recycling endosomal-independent. PlosOne, 9(3), e92013 doi:10.1371/journal.pone.0092013 (16 pages) (2014) *Co-PIs.
- Hamilton, K.L. and A. Grant Butt. Glucose transport into everted sacs of the small intestine of mice. Advances Physiology Education, 37:415-426, (2013).
- Hamilton, K.L. Cyclosporine and lovastatin – the good and the bad, but who will be the winner? American Journal Physiology – Renal Physiology, 305:F643-F644, (2013).
- Hamilton, K.L. Robert K. Crane: Na+-glucose cotransporter to cure? Front. Physiol. 4:53. doi:103389/fphys.2013.00053 (2013).
- Balut, C.M. Hamilton, K.L, and Devor, D.C. Trafficking of intermediate (KCa3.1) and small (KCa2.x) conductance, Ca2+-activated K+ channels: A novel target for medicinal chemistry efforts? ChemMedChem 7: 1741-1755, (2012).
- Hamilton, K.L. and Pao, A.C. β-Pix is a new player in renal physiology. Front. Physiol., 3:268. doi: 10.3389/fphys.2012.00268 (2012).
- Hamilton, K.L. BLAP-tags, TUBEs and DUB-Chips: Combined novel technologies will advance molecular epithelial physiology. Front. Physiol., 3:137, doi:10.3389/ fphys.2012.00137 (2012)
- Hamilton, K.L. and Devor D.C. Basolateral membrane K+ channels of renal epithelia. Am. J. Physiol. Renal Physiol. 302:F1069-F1081. (2012).
- Devor, D.C., Bertuccio C.A. and Hamilton, K.L. (2016) KCa3.1 in epithelia. Chapter 20. In Ion Channels and Transporters in Health and Disease. 1st Edition, Physiology in Health and Disease K.L. Hamilton and D.C. Devor (eds.). pp 659-705. Springer-Verlag, (New York), DOI: 10.1007/978-1-4939-3366-2_20, (2016)
- Nickerson D.P., Hamilton, K.L. and Hunter, P.J. (2016) Mathematical modeling of epithelial ion transport. Chapter 6, In Ion Channels and Transporters in Health and Disease. 1st Edition, Physiology in Health and Disease. K.L. Hamilton and D.C. Devor (eds.). pp 265-278. Springer-Verlag, (New York), DOI: 10.1007/978-1-4939-3366-2)_6, (2016)
- Convener of PHSL 233
- HUBS 192 - Human Biology Studies II
- PHSL 233 - Cellular Gastrointestinal and Renal Physiology
- PHSL 343 - Cellular and Epithelial Physiology
- PHSL 345 - Physiological Aspects of Health and Disease
- PHSL 473 - Cellular Physiology
- Med 2 - Physiology (Case Studies)